Generation of DNA-Free Escherichia coli Cells by 2-Aminopurine Requires Mismatch Repair and Nonmethylated DNA

  1. Ivan Matic1,*,
  2. Damian Ekiert2,
  3. Miroslav Radman1, and
  4. Masamichi Kohiyama2

+ Author Affiliations

  1. 1INSERM U571, Faculté de Médecine Necker-Université Paris V, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
  2. 2Institut Jacques Monod-CNRS, Universités Paris 6 & 7, 2 place Jussieu, 75251 Paris Cedex 05, France

 

 

ABSTRACT

Undirected mismatch repair initiated by the incorporation of the base analog 2-aminopurine kills DNA-methylation-deficient Escherichia coli dam cells by DNA double-strand breakage. Subsequently, the chromosomal DNA is totally degraded, resulting in DNA-free cells.

 

 

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